Genome Modification Facility

MCB's Genome Modification Facility (GMF) provides transgenic, gene targeting, and other services. The GMF performs microinjections of DNA into fertilized embryos to generate transgenic mice, DNA transfection into ES cells for the creation of recombinant ES cell clones, injection of gene-targeted ES cells into host blastocysts to generate gene knock-out or knock-in mice, teratoma formation studies, and other related ES cell-based services. The GMF also generates preps for gRNA validations, and performs microinjections of TALENs or CRISPR/Cas9 and sgRNA as well as DNA donors into mouse zygotes for gene editing projects. Other services include cryopreservation of mouse sperm and embryos, in vitro fertilization, recovery of cryopreserved mouse sperm and embryos, rederivation of pathogen free mouse lines, and establish new ES cell lines from wild type and mutant mice with a variety of genetic backgrounds.

• Director:  Lin Wu

Please visit the Genome Modification Facility website for more information.